Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine

The study of cell kinetics has traditionally involved the use of radioactive precursors of DNA, such as tritiated thymidine ((3)HTdR), and autoradiography to detect their incorporation into DNA. These techniques have provided detailed knowledge of cell kinetics in both in vitro and in vivo experimental systems. The technique, however, is time consuming and arduous and is not readily applicable to human tumor research because of ethical problems involved in incorporation of a radioisotope into DNA.