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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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PASADENA hyperpolarized 13C phospholactate.

Roman V Shchepin1, Aaron M Coffey, Kevin W Waddell

  • 1Department of Radiology, Vanderbilt University Institute of Imaging Science (VUIIS), Nashville, Tennessee 37232, USA.

Journal of the American Chemical Society
|February 23, 2012
PubMed
Summary
This summary is machine-generated.

Potassium 1-(13)C-phosphoenolpyruvate was hyperpolarized to potassium 1-(13)C-phospholactate using parahydrogen and synthesis allows dramatically enhanced nuclear alignment (PASADENA). This achieved a 4000-fold sensitivity enhancement for (13)C polarization at 3 T.

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Hyperpolarization Techniques
  • Chemical Physics

Background:

  • Nuclear spin hyperpolarization significantly enhances NMR signal sensitivity.
  • Parahydrogen-induced polarization (PHIP) is a key method for hyperpolarization.
  • Developing efficient hyperpolarization methods is crucial for advancing NMR applications.

Purpose of the Study:

  • To demonstrate the hyperpolarization of potassium 1-(13)C-phospholactate from potassium 1-(13)C-phosphoenolpyruvate.
  • To report the (13)C spin-lattice relaxation time (T1) of the hyperpolarized product.
  • To quantify the sensitivity enhancement achieved using the PASADENA technique.

Main Methods:

  • Utilized the PASADENA (Parahydrogen and Synthesis Allows Dramatically Enhanced Nuclear Alignment) technique for hyperpolarization.
  • Employed molecular hydrogenation of a fully protonated precursor.
  • Performed NMR spectroscopy at 3 Tesla to measure polarization levels and relaxation times.

Main Results:

  • Successfully converted potassium 1-(13)C-phosphoenolpyruvate to hyperpolarized potassium 1-(13)C-phospholactate.
  • Achieved a (13)C T1 relaxation time of 36 seconds for the hyperpolarized product.
  • Demonstrated a 1% (13)C polarization level, yielding a ~4000-fold sensitivity enhancement at 3 T.

Conclusions:

  • The PASADENA technique enables significant hyperpolarization of specific molecules.
  • This method offers a substantial increase in NMR sensitivity for (13)C observation.
  • The reported findings represent a proof-of-principle for enhanced NMR spectroscopy using this approach.