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Engineering cellular trafficking via glycosyltransferase-programmed stereosubstitution.
1Department of Dermatology, Brigham & Women's Hospital, Boston, Massachusetts, USA. Rsackstein@rics.bwh.harvard.edu
Annals of the New York Academy of Sciences
|February 23, 2012
Summary
Targeting cell trafficking, this study highlights how enhancing the hematopoietic cell E-/L-selectin ligand (HCELL) via glycosyltransferase-programmed stereosubstitution improves E-selectin-mediated cell delivery for therapeutics.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Cell trafficking to inflamed tissues requires initial endothelial attachment, a process mediated by E-selectin.
- E-selectin, an inducible endothelial molecule, binds sialofucosylated glycans on cell surface ligands.
- Hematopoietic cell E-/L-selectin ligand (HCELL), a CD44 glycoform, is the primary E-selectin ligand on human cells.
Purpose of the Study:
- To review the biological role of HCELL/E-selectin interactions in cell migration.
- To discuss the application of glycosyltransferase-programmed stereosubstitution (GPS) for glycoengineering HCELL expression.
Main Methods:
- Focus on the biology of HCELL/E-selectin interactions.
- Discussion of glycosyltransferase-programmed stereosubstitution (GPS) technology for glycoengineering.
Main Results:
- E-selectin-mediated cell adhesion is crucial for cell trafficking to inflammatory sites.
- Glycoengineering HCELL using GPS enhances its binding to E-selectin.
- This exoglycosylation technology improves E-selectin-dependent vascular delivery without affecting cell viability or phenotype.
Conclusions:
- HCELL/E-selectin interactions are key regulators of cell migration.
- Glycosyltransferase-programmed stereosubstitution (GPS) offers a novel approach for enhancing HCELL expression.
- This glycoengineering strategy facilitates E-selectin-mediated vascular delivery for cell-based therapeutics.
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