Related Experiment Video
Updated: May 24, 2026
11:02
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder
1Institute of Cancer, College of MVLS, University of Glasgow, Western Infirmary, Glasgow G11 6NT, UK.
British Journal of Cancer
|February 23, 2012
Summary
Activated Src family kinases and downstream proteins indicate a good prognosis in bladder cancer, unlike other tumors. This activation positively correlates with RhoGD12 expression, suggesting a favorable outcome for patients.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Investigating signaling pathways in bladder cancer is crucial for understanding tumor progression.
- Src family kinases (SFKs), focal adhesion kinase (FAK), caveolin-1 (Cav-1), and RhoGD12 are implicated in various cancers.
Purpose of the Study:
- To evaluate the expression and activity of SFKs, FAK, Cav-1, and RhoGD12 in bladder cancer patients.
- To determine the prognostic significance of these molecules in transitional cell carcinoma of the bladder.
Main Methods:
- Immunohistochemical staining was performed on tumor samples from 58 bladder cancer patients.
- Expression levels of c-Src, phosphorylated Src (Y419), dephosphorylated Src (Y527), phosphorylated FAK (Y861), Cav-1, and RhoGD12 were quantified using the weighted histoscore method.
Main Results:
- High membrane expression of dephosphorylated Src (Y527) and phosphorylated Src (Y419) correlated with increased cancer-specific survival.
- Elevated phosphorylated FAK (Y861) at the membrane also showed association with better survival.
- Membrane Src Y419 expression was an independent prognostic factor in multivariate analysis.
Conclusions:
- Contrary to findings in other solid tumors, activated SFKs and downstream signaling proteins are associated with a favorable prognosis in bladder cancer.
- Activated Src demonstrates a positive relationship with RhoGD12 expression, suggesting a potential interplay in bladder tumor biology.
Related Concept Videos
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:24Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
01:21Cell Polarization by Rho Proteins
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
01:32Cytoskeletal Coordination in Cell Migration
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...