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14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
No decrease in susceptibility to NVC-422 in multiple-passage studies with methicillin-resistant Staphylococcus
Louisa D'Lima1, Lisa Friedman, Lu Wang
1NovaBay Pharmaceuticals, Inc, Emeryville, California, USA..
Antimicrobial Agents and Chemotherapy
|February 23, 2012
Summary
NVC-422 showed no increased resistance in bacteria after serial passages. However, ciprofloxacin and other antibiotics like mupirocin developed significant resistance, with no cross-resistance observed for NVC-422.
Area of Science:
- Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Antibiotic resistance is a growing global health threat.
- Development of novel antimicrobial agents with low resistance potential is crucial.
Purpose of the Study:
- To evaluate the in vitro resistance development potential of NVC-422 against common bacterial pathogens.
- To compare the resistance profile of NVC-422 with existing antibiotics.
Main Methods:
- Serial passage experiments were conducted on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus (including MRSA).
- Minimum Inhibitory Concentrations (MICs) for NVC-422, ciprofloxacin, mupirocin, fusidic acid, and retapamulin were determined after passages.
Main Results:
- No significant increase in NVC-422 MICs was observed for any tested bacteria after serial passages.
- Ciprofloxacin MICs increased significantly (256-fold for E. coli, 32-fold for P. aeruginosa and S. aureus).
- MRSA developed substantial resistance to mupirocin (64-fold), fusidic acid (256-fold), and retapamulin (16-fold), with no cross-resistance to NVC-422.
Conclusions:
- NVC-422 demonstrates a low propensity for in vitro resistance development.
- Existing antibiotics like ciprofloxacin, mupirocin, fusidic acid, and retapamulin showed significant resistance development in this study.
- NVC-422 represents a promising candidate for combating resistant bacterial infections.
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