Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test
Zablon K Njiru1, Dorothy Yeboah-Manu, Timothy P Stinear
1School of Veterinary Sciences, The University of Queensland, Gatton Campus, Gatton, Queensland, Australia. z.njiru@uq.edu.au
Journal of Clinical Microbiology
|February 24, 2012
Abstract:
This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.
