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Updated: May 24, 2026
The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
Published on: February 5, 2014
Identification of DNA-protein complexes using an improved, combined western blotting-electrophoretic mobility shift
Klaus Deckmann1, Florian Rörsch, Gerd Geisslinger
1Pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt/Main, Germany. Klaus-Deckmann@gmx.de
Abstract:
Electrophoretic Mobility Shift Assays (EMSAs) are used to detect DNA-protein interactions. With this type of assay it is difficult to distinguish between specific and non-specific DNA-protein complexes or to define which protein binds to the DNA. Here we describe a novel Western blot-combined EMSA (WEMSA) variant for a fluorescence imaging system which permits easy identification of specific DNA-protein-complexes. This method also allows investigation of several DNA-protein complexes in parallel. We have identified and distinguished clearly between the SP1- and EGR1-DNA-protein complexes which exhibit overlapping binding to the GC-BOX of the mPGES-1 promoter.
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