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A new apparatus for studying the effect of hydrostatic pressure on cells in culture : application to osteoblastic
L Vergne1, A Meunier, M Adolphe
1Laboratoire de Recherches Orthopédiques, Université Paris VII Denis Diderot, URA CNRS 1432, Faculté de Médecine Lariboisière Saint-Louis, 10 Avenue de Verdun, 75010, Paris, France.
Abstract:
Although mechanical stresses have long been recognized as an important factor in the regulation of bone remodeling, the mechanism underlying this effect has remained obscure. A number of methods have been devised to apply forces to bone tissues and bone-derived cells in order to investigate the biochemical results of mechanical stimuli. In this paper we report a method for applying a well controlled cyclic hydrostatic pressure on cultured ROS 17/2.8 osteoblastic lineage cells. This technique allows the investigation of the true frequency response of cells. Hydrostatic pressure with a 1 Hz frequency decreases alkaline phosphatase activity of confluent osteoblastic-like cells (ROS 17/2.8).
