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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: May 24, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Screening for miRNA expression changes using quantitative PCR (Q-PCR).

Richard N Armstrong¹, Hilary A A Colyer, Ken I Mills

¹Haematology Center for Cancer Research and Cell Biology Belfast, Queen's University Belfast, Belfast, Northern Ireland, UK. rarmstrong20@qub.ac.uk

Methods in Molecular Biology (Clifton, N.J.)

|February 24, 2012

Summary

This summary is machine-generated.

This study details the Applied Biosystems TaqMan Array microRNA Card Set 3.0 for profiling microRNA (miRNA) expression. The method uses Real-Time PCR (Q-PCR) to measure genome-wide miRNA expression changes in RNA samples.

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Last Updated: May 24, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

Molecular Biology
Genomics

Background:

MicroRNAs (miRNAs) are small non-coding RNAs that play crucial roles in gene regulation.
Accurate profiling of miRNA expression is essential for understanding biological processes and disease mechanisms.

Purpose of the Study:

To describe the application of the Applied Biosystems TaqMan Array microRNA Card Set 3.0 for comprehensive miRNA expression analysis.
To evaluate the utility of this array set for genome-wide miRNA profiling.

Main Methods:

Utilized Applied Biosystems TaqMan Array microRNA Card Set 3.0, comprising two 384-well arrays (Array A and Array B).
Employed Real-Time PCR (Q-PCR) with specific oligonucleotide assays to quantify individual miRNA expression levels in RNA samples.
Array A is designed for profiling highly expressed and well-characterized miRNAs.

Main Results:

The TaqMan Array microRNA Card Set 3.0 enables the measurement of 754 distinct miRNAs.
The array format is suitable for genome-wide miRNA expression profiling due to the relatively small number of miRNAs compared to protein-coding genes.
miRBase release 16 annotates 1,048 mature miRNAs, with the array covering a significant portion.

Conclusions:

The Applied Biosystems TaqMan Array microRNA Card Set 3.0 provides a robust platform for quantitative miRNA expression profiling.
This method facilitates the study of genome-wide miRNA expression changes, contributing to a deeper understanding of miRNA function in biological systems.