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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 24, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Detecting DNA methylation using the methylated CpG island amplification and microarray technique.

Hilary A A Colyer¹, Margaret Dellett, Ken I Mills

¹Haematology, Centre for Cancer Research and Cell Biology Belfast, Queen's University Belfast, Belfast, Northern Ireland, UK. hcolyer01@qub.ac.uk

Methods in Molecular Biology (Clifton, N.J.)

|February 24, 2012

Summary

This summary is machine-generated.

Methylated CpG island amplification and microarray (MCAM) quantifies DNA methylation using a two-color array. This high-throughput technique allows simultaneous processing of multiple samples for efficient data acquisition.

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Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

DNA methylation is a critical epigenetic mechanism influencing gene expression.
Accurate quantification of DNA methylation is essential for understanding various biological processes and diseases.

Purpose of the Study:

To describe the Methylated CpG island amplification and microarray (MCAM) technique.
To highlight MCAM's utility in quantifying DNA methylation.

Main Methods:

MCAM is a two-color array technique.
It involves hybridizing equimolar amounts of treated versus control cell-line DNA to an array platform.
Sample preparation, hybridization, and scanning are completed within one week.

Main Results:

MCAM enables the quantification of DNA methylation.
The technique allows for high-throughput processing and data acquisition.
Simultaneous preparation of multiple samples is feasible.

Conclusions:

MCAM is an efficient method for DNA methylation analysis.
The technique supports high-throughput studies in epigenetics.
MCAM facilitates robust data acquisition for methylation profiling.