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Related Experiment Video

Updated: May 24, 2026

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Published on: March 3, 2017

Plasma proteomic profiling in HIV-1 infected methamphetamine abusers.

Gwenael Pottiez1, Teena Jagadish, Fang Yu

  • 1Department of Pharmacology and Experimental Neuroscience, Omaha, Nebraska, United States of America.

Plos One
|February 24, 2012
PubMed
Summary

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Methamphetamine (METH) use alters plasma protein levels in HIV-infected individuals, independent of HIV infection itself. These changes impact complement, coagulation, and oxidative stress pathways, indicating METH

Area of Science:

  • Biochemistry
  • Immunology
  • Infectious Diseases

Background:

  • Human immunodeficiency virus (HIV) infection is associated with immune dysregulation.
  • Methamphetamine (METH) use is prevalent among populations at risk for or living with HIV.
  • The combined effects of HIV and METH on plasma proteomes are not well understood.

Purpose of the Study:

  • To investigate the impact of ongoing methamphetamine use on plasma protein expression in HIV-infected individuals.
  • To differentiate protein changes attributable to METH use from those caused by HIV infection.

Main Methods:

  • Plasma samples were collected from four groups: HIV-positive with persistent METH use, HIV-positive with short-term METH abstinence, HIV-positive with long-term METH abstinence, and HIV-negative with no METH history.

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Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
06:46

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Published on: January 5, 2016

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Last Updated: May 24, 2026

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
10:24

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Published on: March 3, 2017

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
06:46

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Published on: January 5, 2016

  • Proteomic analysis was performed to identify and quantify plasma proteins.
  • Statistical analysis was used to identify significant changes in protein expression between groups and over time.

    • Main Results:

    • Out of 390 identified proteins, 28 showed significant expression changes in HIV-positive individuals with persistent METH use.
    • These changes were observed over two study visits and were not attributable to HIV infection alone.
    • Affected proteins were primarily involved in complement pathways, coagulation cascades, and oxidative stress responses.

    Conclusions:

    • Persistent methamphetamine use significantly alters plasma protein profiles in HIV-infected individuals.
    • These alterations affect key biological pathways, including immune response and hemostasis.
    • Continuous METH use represents an unstable physiological condition with measurable proteomic consequences.