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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

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Fluorescence detection methods for microfluidic droplet platforms
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Published on: December 10, 2011

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter.

Ting-Hsiang Wu1, Yue Chen, Sung-Yong Park

  • 1Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, Rm 37-138, Los Angeles, CA 90095, USA.

Lab on a Chip
|February 25, 2012
PubMed
Summary

We developed a high-speed, high-purity pulsed laser triggered fluorescence activated cell sorter (PLACS). This innovative cell sorter achieves rapid throughput and high purity for efficient cell analysis and sorting applications.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Sorting

Background:

  • Traditional cell sorters face limitations in speed and purity.
  • Microfluidic devices offer potential for enhanced cell manipulation.

Purpose of the Study:

  • To develop a high-speed and high-purity pulsed laser activated cell sorter (PLACS).
  • To achieve high throughput and purity for mammalian cell sorting.

Main Methods:

  • Utilized a pulsed laser to trigger fluorescence activated cell sorting.
  • Employed a unique sorting mechanism generating explosive vapor bubbles in a microfluidic PDMS channel.
  • Achieved fast switching (30 μs) and small perturbation volume (~90 pL).

Main Results:

  • Sorting throughput up to 20,000 mammalian cells/s.
  • Achieved 37% sorting purity in standard mode.
  • Demonstrated 90% cell viability in enrichment mode and >90% purity in high purity mode at 1500 cells/s or 3000 beads/s.

Conclusions:

  • The developed PLACS system offers significant advancements in cell sorting speed and purity.
  • The laser-triggered vapor bubble mechanism enables efficient and precise cell sorting.
  • This technology has potential applications in various biological and medical fields requiring high-throughput cell analysis.