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Published on: June 25, 2017
[Endothelial dysfunction induced by high glucose is associated with decreased ATP-binding cassette transporter G1
Jiahong Xue1, Xiaolin Niu, Jin Wei
1Department of Cardiovascular Medicine, Xi'an Jiaotong University College of Medicine, Xi'an, China. xjh1224@163.com
Objective:
To investigate the role of ATP-binding cassette transporter G1 (ABCG1) in endothelial dysfunction induced by high glucose.
Methods:
Human aortic endothelial cells (HAECs) were incubated in the presence of 5.6 or 30 mmol/L glucose for 24-72 h with or without a 2-h pretreatment with the LXR agonist 22(R)-hydroxycholesterol. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCG1; the intracellular cholesterol efflux and endothelial nitric oxide synthase (eNOS) activity were measured by scintillation counting.
Results:
High glucose time-dependently suppressed ABCG1 expression and cholesterol efflux to HDL in HAECs. High glucose also decreased eNOS activity. ABCG1 down-regulation induced by high glucose, along with decreased cholesterol efflux and eNOS activity, was abolished by treatment of the cells with the LXR agonist.
Conclusion:
Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression.
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