Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Drug-Delivering-Drug" nanocomplexes reinforce cytosolic nucleic acid sensing for broad-spectrum antiviral activity in fish.

Journal of nanobiotechnology·2026
Same author

Epitope-Imprinted Polymers: Fabrication Technologies and Emerging Applications.

Analytical chemistry·2026
Same author

Crosslinker nanocarrier-based intratumoral delivery for protein complex mapping in mitochondria of live tumor-bearing mice.

Chemical science·2026
Same author

Automated strain-to-peptide conversion: a high-throughput proteome analysis platform empowering rational design of microbial cell factories.

Bioresource technology·2026
Same author

Dual-Responsive Nanogel for Mitochondria-Targeted Delivery and Release of Cross-Linkers to Decipher In Vivo Protein Conformations and Interactions.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Metaproteomic Insights into Bioenergy Conversion Enzymes of Bathypelagic Microbial Communities in the South China Sea.

Journal of proteome research·2025
Same journal

Mulberry-like Zn-MOF/ZnS heterojunction constructed for high-sensitivity electrochemical simultaneous determination of naphthol isomers.

Analytical and bioanalytical chemistry·2026
Same journal

Analyses of dextroamphetamine and its metabolites in human urine by capillary electrophoresis with diode array and capacitively coupled contactless conductivity detection (CE-DAD-C<sup>4</sup>D).

Analytical and bioanalytical chemistry·2026
Same journal

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Analytical and bioanalytical chemistry·2026
Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026
Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Protein-imprinted materials: rational design, application and challenges.

Kaiguang Yang1, Lihua Zhang, Zhen Liang

  • 1National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China.

Analytical and Bioanalytical Chemistry
|February 28, 2012
PubMed
Summary
This summary is machine-generated.

Protein imprinting creates artificial receptors that mimic antibodies for various applications. This review details their preparation, highlighting advantages like stability and low cost, and discusses challenges and future directions.

More Related Videos

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization
07:28

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization

Published on: February 18, 2022

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-&#181;CP) and Sequential Nucleophilic Substitution
08:23

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

Related Experiment Videos

Last Updated: May 24, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization
07:28

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization

Published on: February 18, 2022

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-&#181;CP) and Sequential Nucleophilic Substitution
08:23

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

Area of Science:

  • Biomaterials Science
  • Chemical Engineering
  • Biotechnology

Background:

  • Protein imprinting offers a method for creating synthetic receptors with high specificity.
  • These protein-imprinted materials serve as viable alternatives to antibodies in diverse applications.
  • Key advantages include stability, reusability, and cost-effectiveness compared to traditional antibodies.

Purpose of the Study:

  • To critically review the rational preparation of protein-imprinted materials.
  • To highlight the diverse and unique applications of these biomimetic receptors.
  • To identify and discuss the inherent challenges in protein imprinting.

Main Methods:

  • Focus on rational design principles for template selection.
  • Analysis of functional monomers, crosslinkers, and polymerization techniques.
  • Review of existing literature on protein imprinting preparation and applications.

Main Results:

  • Established guidelines for selecting optimal components for protein imprinting.
  • Demonstrated broad applicability in chemical sensors, chromatography, artificial enzymes, proteomics, and tissue engineering.
  • Identified key challenges such as template acquisition and binding heterogeneity.

Conclusions:

  • Protein imprinting is a powerful technique for developing robust biomimetic receptors.
  • Careful consideration of preparation parameters is crucial for successful imprinting.
  • Addressing inherent challenges will further expand the utility of protein-imprinted materials.