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Updated: May 24, 2026

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

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Exploring bacterial insertion sequences with ISfinder: objectives, uses, and future developments.

P Siguier¹, A Varani, J Perochon

¹Centre National de la Recherche Scientifique, LMGM, Toulouse, France.

Methods in Molecular Biology (Clifton, N.J.)

|February 28, 2012

Summary

This summary is machine-generated.

ISfinder is a database for prokaryotic insertion sequences (ISs). It now offers tools like ISbrowser for visualizing IS copies and automated genome annotation for high-quality results.

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Area of Science:

Bioinformatics
Genomics
Microbiology

Background:

Insertion sequences (ISs) are mobile genetic elements crucial for bacterial genome evolution.
Comprehensive databases are needed for efficient analysis of IS elements across prokaryotic genomes.

Purpose of the Study:

To introduce the ISfinder database and its integrated software tools.
To facilitate the visualization and annotation of insertion sequences in prokaryotic genomes.

Main Methods:

Utilizing the ISfinder database for comprehensive storage of prokaryotic insertion sequences.
Employing ISbrowser software for visualizing complete and partial IS copies within whole genomes.
Implementing Insertion Sequence Semi-automatic Genome Annotation for high-quality genome annotation.

Main Results:

ISfinder provides a centralized resource for prokaryotic insertion sequences.
ISbrowser enables effective visualization of IS elements in genomic contexts.
The annotation tool supports high-quality genome characterization regarding IS elements.

Conclusions:

ISfinder and its associated software offer powerful tools for IS element research.
These resources enhance the study of genome dynamics and mobile genetic elements in prokaryotes.