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Updated: May 24, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies
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Ac-Ds solutions for rice insertion mutagenesis.

Emmanuel Guiderdoni1, Pascal Gantet

  • 1CIRAD, UMR AGAP, Montpellier Cedex 5, France.

Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2012
PubMed
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Researchers developed stable Ds insertion mutations in rice using the maize Ac-Ds transposable element system. This method aids in identifying gene functions in the model monocotyledonous plant.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Rice serves as a model organism for monocotyledonous plants.
  • Identifying gene function is crucial following the completion of the rice genome sequence.
  • Insertion mutagenesis is a key strategy for gene discovery.

Purpose of the Study:

  • To introduce a functional Ac-Ds transposable element system into rice.
  • To generate and select Ds insertion mutations in the rice genome.
  • To create stable mutations for functional genomics studies.

Main Methods:

  • Utilizing single- or double-component T-DNA constructs.
  • Employing Agrobacterium-mediated transformation for gene insertion.
  • Leveraging the maize Ac-Ds system for targeted mutagenesis in rice.

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Last Updated: May 24, 2026

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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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Published on: September 2, 2019

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Main Results:

  • Successfully introduced the Ac-Ds system into the rice genome.
  • Generated and selected various Ds insertion mutations.
  • Demonstrated the stability of induced mutations upon removal of the Ac transposase source.

Conclusions:

  • The Ac-Ds system is effective for generating stable insertion mutations in rice.
  • This approach facilitates the functional analysis of rice genes.
  • The generated mutants are valuable resources for the scientific community.