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Updated: May 24, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Separation of natural products by countercurrent chromatography
James B McAlpine1, J Brent Friesen, Guido F Pauli
1Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA. mcalpine@uic.edu
Abstract:
Countercurrent Chromatography (CCC) provides the natural product chemist with a high-resolution separatory method, which is uniquely applicable to sensitive (unstable) compounds and which allows virtually quantitative recovery of the load sample. Different instruments use different means of retaining a stationary liquid phase. The solvent system (SS) can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in choice of an appropriate SS.
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