Related Experiment Video
Updated: May 24, 2026

Strategies to Enhance Cultivation of Anaerobic Bacteria from Gastrointestinal Tract of Chicken
Published on: May 10, 2024
Leucobacter denitrificans sp. nov., isolated from cow dung
Hang-Yeon Weon1, Rangasamy Anandham, Tomohiko Tamura
1Korean Agricultural Culture Collection (KACC), National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Republic of Korea.
Abstract:
The bacterial strain M1T8B10(T) was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stain-positive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9(T) (98.7%), Leucobacter iarius 40(T) (98.4%), and Leucobacter komagatae JCM 9414(T) (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C(15:0), iso-C(16:0), and anteiso-C(17:0). The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10(T) (=KACC 14055(T) =NBRC 106309(T)).
Related Concept Videos
Microbes in the Production of Fermented Foods
Microbial Leaching
Microbiota of the Large Intestine
Bacterial Phylum Spirochaetes
Bacterial Phylum Firmicutes
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.

