Kineococcus anabasis sp. nov., isolated from seeds of Anabasis brevifolia

A novel actinomycete, designated as NUM-3379^T, was isolated from seeds of Anabasis brevifolia collected from Urgun Soum, Dornogovi Province, Mongolia. The cells of the strain were aerobic, Gram-positive, motile, non-spore-forming, and coccus-shaped. 16S rRNA gene sequence analysis indicated that strain NUM-3379^T belonged to the genus Kineococcus and showed the highest similarity to Kineococcus glutinatus DSM 26692^T and Kineococcus xinjiangensis DSM 22857^T, with both sharing 98.87% sequence similarity. The chemotaxonomic characteristics of the strain NUM-3379^T were consistent with those of the genus Kineococcus. The diagnostic diamino acid for peptidoglycans was meso-diaminopimelic acid. The whole-cell sugars included glucose, galactose, mannose, and arabinose. The major menaquinone was MK-9(H₂). The polar lipid profile was comprised of phosphatidylglycerol, diphosphatidylglycerol, and two unidentified phospholipids. The major fatty acids were anteiso-C_15:0 and iso-C_17:0 3-OH. In contrast, digital DNA-DNA hybridization and average nucleotide identity values revealed low relatedness between strain NUM-3379^T and other type strains of the genus Kineococcus. Furthermore, strain NUM-3379^T exhibited several phenotypic properties that distinguished it from other closely related species. Based on the genotypic and phenotypic data, strain NUM-3379^T represents a novel species within the genus Kineococcus, for which the name Kineococcus anabasis sp. nov. is proposed. The type strain is NUM-3379^T (=NBRC 117252^T = TBRC 21100^T).