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Updated: May 24, 2026

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

[Interaction of SerpinB5 and MAFbx in gastric cancer cell and its action site]

Ke-feng Lei1, Yi-fei Wang, Qun-qun Wang

  • 1Department of Surgery, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Zhonghua Wei Chang Wai Ke Za Zhi = Chinese Journal of Gastrointestinal Surgery
|February 28, 2012
PubMed
Abstract

Insights

Serine protease inhibitor B5 (SerpinB5) interacts with MAFbx in gastric cancer cells. Key amino acids PRO261, ASN361, and LYS362 were identified as crucial for this interaction.

Area of Science:

  • Molecular biology
  • Oncology
  • Protein-protein interactions

Background:

  • Gastric cancer is a significant global health concern.
  • Understanding protein interactions is crucial for identifying therapeutic targets.

Purpose of the Study:

  • To investigate the interaction between SerpinB5 and MAFbx in gastric cancer.
  • To pinpoint the specific amino acid residues involved in this interaction.

Main Methods:

  • Yeast two-hybrid screening and co-immunoprecipitation were used to validate the interaction.
  • RNA interference and gene transfection were employed to study gene expression.
  • Homology modeling and molecular docking (Autodock4.0) were utilized to analyze interaction sites.

Main Results:

  • The interaction between SerpinB5 and MAFbx was confirmed.
  • MAFbx expression levels were found to correlate with SerpinB5 expression.
  • PRO261, ASN361, and LYS362 were identified as critical residues for the interaction.

Conclusions:

  • SerpinB5 directly interacts with MAFbx within gastric cancer cells.
  • The identified amino acids (PRO261, ASN361, LYS362) represent potential binding sites for therapeutic intervention.

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