High performance liquid chromatography determination of fatty acids in drying oils following lipase action

This paper describes a quantitative analytical procedure to determine the fatty acid composition in drying oils like linseed, walnut and poppy seed. The procedure required the enzymatic hydrolysis of the oil triacylglycerol families by the action of Candida rugosa lipase. The fatty acids (FFAs) produced (linolenic, myristic, linoleic, palmitic, oleic and stearic) were extracted with n-heptane and derivatized with α-bromoacetophenone. Their separation and quantitative determination were performed by high-performance liquid chromatography employing a C18 column and an isocratic elution method coupled to ultraviolet detection. The analytical enzymatic procedure is sensitive for < 0.5 µg/mL of FFAs in a reduced sample of 0.1 mg of drying oil.