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Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Updated: May 24, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Sheathless size-based acoustic particle separation.

Rasim Guldiken¹, Myeong Chan Jo, Nathan D Gallant

¹Department of Mechanical Engineering, University of South Florida, Tampa, FL 33620, USA. guldiken@usf.edu

Sensors (Basel, Switzerland)

|February 28, 2012

Summary

This summary is machine-generated.

This study introduces a sheathless microfluidic platform using acoustic waves for particle separation. This innovative method avoids sheath flow, simplifying device design and improving efficiency for biomedical applications.

Keywords:

separation sheathless size-based standing surface acoustic wave

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Published on: March 6, 2016

Area of Science:

Biotechnology
Microfluidics
Acoustic Physics

Background:

Particle separation is crucial for biological and biomedical applications.
Traditional flow-based methods require sheath flow, leading to dilution and complex designs.
Sheath flow's limitations hinder efficiency and necessitate precise flow control.

Purpose of the Study:

To present a novel microfluidic platform for sheathless particle separation.
To utilize standing surface acoustic waves (SSAWs) for particle manipulation.
To overcome the limitations of conventional sheath-flow-dependent separation techniques.

Main Methods:

Developed a microfluidic device integrating sheathless flow focusing and separation.
Employed SSAWs to align particles and induce size-based separation.
Investigated the impact of acoustic forces on particles of different sizes.

Main Results:

Successfully demonstrated sheathless separation of particles with different sizes (3 μm vs. 10 μm, and 3 μm vs. 5 μm).
Showcased simultaneous particle alignment and separation within a single device.
Analyzed the influence of input power, flow rate, and particle concentration on separation efficiency.

Conclusions:

The developed microfluidic platform offers an efficient sheathless approach for particle separation.
This technology eliminates the need for sheath flow, simplifying device operation and design.
Potential applications include lab-on-a-chip systems and integrated biological/biomedical devices.