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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 24, 2026

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

Published on: October 28, 2022

What has proteomics taught us about Leishmania development?

Polina Tsigankov¹, Pier Federico Gherardini, Manuela Helmer-Citterich

¹Technion-Israel Institute of Technology, Haifa 32000, Israel.

|February 29, 2012

Summary

This summary is machine-generated.

Leishmania parasites adapt to host environments through genetically programmed changes. Proteomics reveals that their differentiation from promastigotes to amastigotes is a strictly coordinated and regulated process.

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Last Updated: May 24, 2026

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

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Published on: October 28, 2022

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

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Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

Area of Science:

Parasitology
Molecular Biology
Cell Biology

Background:

Leishmania are protozoan parasites residing in sand flies and macrophages.
They undergo differentiation between these hosts, adapting gene expression.
Axenic cultures mimic host environments for studying this development.

Purpose of the Study:

To summarize current understanding of Leishmania differentiation.
To highlight the role of proteomics in elucidating this process.

Main Methods:

Axenic culture systems for Leishmania promastigote to amastigote differentiation.
Transcriptome and proteomic analyses of differentiated stages.

Main Results:

Leishmania differentiation involves genetically programmed expression changes.
Proteomic analysis revealed coordinated and regulated development, contrary to initial observations of variability.
This highlights molecular mechanisms of host-parasite interaction.

Conclusions:

Axenic cultures are valuable tools for studying Leishmania development.
Proteomics provides crucial insights into the regulated nature of Leishmania differentiation.
Understanding differentiation is key to targeting parasite survival.