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[Establish efficiently transformation system of the Isatis indigotica]
Yan-Xia Sun1, Yue-Hua Wang, Xing-Hua Gou
1The Key Laboratory of Medicinal and Edible Plants Resources Exploitation of Chengdu University, Chengdu 610106, China. sunyanxia1976@cdu.edu.cn
Objective:
To build up a method of efficiently transforming Isatis indigotica with the Agrobacterium.
Methods:
Two kinds of Agrobocterium: ATCC 15834 and RI1601 were used to treat different fraction of Isatis indigotica. Explored the effect of cocultured, different antibiotic concentration and the media on multiply the hairy roots.
Results:
The explants with out coculture had had higher survival rate rooting rate and earlier sprout time. After ultrasonic treatment of plant, the indacement rate was two times than that of untreated one; The best antibiotic concentration was 400 mg/L; The proportion of the grow speed of the hairy root in the liquid culture media was 2 - 3 times than that of solid culture media, and 37 times of common roots.
Conclusion:
The method is useful for establishing an efficiently transformatiem system of Isatis indigotica by Agrobacterium.
