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[Separation and purification process of total flavones from four-leaf diet decoction]
Qiang Wei1, Wei Yin, Wen-Jing Zhang
1Pharmacology College, Anhui Xinhua University, Hefei 230088, China. weiqiang509@sina.com
Objective:
To investigate purification process of total flavonoids from four-leaf diet decoction with macroreticular resin.
Methods:
By using the static absorption tests and dynamic absorption tests with different types of macroreticular resins, the better parameters were found and the orthogonal design were used in purified craft.
Results:
AB-8 resin showed the best absorption property with the following technological conditions: the ratio of resin (g) - extract (mL) is 1.2: 1, the ratio of column diameter to height was 1: 12, then washed with water to colorless, and the volume of 80% ethanol was 7 BV.
Conclusion:
The process is stable and the purity of total flavones is up to 85%.
