[Study on the quality standard of danmo capsule]

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Herbal Medicine Quality Control

Background:

• Danmo capsule is a traditional herbal medicine requiring standardized quality control.
• Ensuring the quality of herbal products is crucial for patient safety and therapeutic efficacy.

Purpose of the Study:

• To establish a quality standard for Danmo capsule.
• To develop and validate analytical methods for its quality assessment.

Main Methods:

• Qualitative identification of Salviae Miltiorrhizae Radix Et Rhizoma and Lonicerae Japonicae Flos using Thin-Layer Chromatography (TLC).
• Quantitative determination of Salvianolic acid B content using High-Performance Liquid Chromatography (HPLC).

Main Results:

• TLC analysis demonstrated clear and well-separated spots, indicating reliable qualitative identification.
• HPLC analysis showed a linear range for Salvianolic acid B from 0.120042 - 2.40084 µg (r = 0.9999), with an average recovery of 103.63% and a relative standard deviation (RSD) of 0.6%.

Conclusions:

• The developed analytical methods are simple, accurate, and reliable.
• These validated methods are suitable for the routine quality control of Danmo capsule.