Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modular Molecular Design and Self-Assembled Nanostructures of Saccharide‑Appended Cyclic Dipeptides for Glycosidase‑Responsive Supramolecular Hydrogels.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Oxidation-Responsive Supramolecular Hydrogels Based on Glucosamine Derivatives with an Aryl Sulfide Group.

Chembiochem : a European journal of chemical biology·2024
Same author

Design of supramolecular hybrid nanomaterials comprising peptide-based supramolecular nanofibers and <i>in situ</i> generated DNA nanoflowers through rolling circle amplification.

Nanoscale·2022
Same author

Construction of a Reduction-responsive DNA Microsphere using a Reduction-cleavable Spacer based on a Nitrobenzene Scaffold.

Chemistry, an Asian journal·2022
Same author

Formation of Supramolecular Nanostructures through in Situ Self-Assembly and Post-Assembly Modification of a Biocatalytically Constructed Dipeptide Hydrazide.

Chemistry (Weinheim an der Bergstrasse, Germany)·2022
Same author

Sulfonamide antibiotics inhibit RNAi by binding to human Argonaute protein 2 PAZ.

Bioorganic & medicinal chemistry letters·2020

Related Experiment Video

Updated: May 24, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Oligonucleotide-templated reactions for sensing nucleic acids.

Aya Shibata1, Hiroshi Abe, Yoshihiro Ito

  • 1Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.

Molecules (Basel, Switzerland)
|March 1, 2012
PubMed
Summary

Oligonucleotide-templated reactions enable nucleic acid sensing through advanced signal amplification and generation systems. This review highlights recent progress in this dynamic field of chemical biology.

More Related Videos

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Related Experiment Videos

Last Updated: May 24, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Oligonucleotide-templated reactions are crucial for sensitive nucleic acid detection.
  • Diverse chemical strategies have been developed for these reactions.
  • Key research areas include signal amplification and signal generation.

Purpose of the Study:

  • To review recent advancements in oligonucleotide-templated reactions.
  • To focus on innovations in signal amplification and generation systems.
  • To provide insights into the future of nucleic acid sensing technologies.

Main Methods:

  • Literature review of recent scientific publications.
  • Analysis of various oligonucleotide-templated reaction chemistries.
  • Categorization of advances based on signal amplification and generation.

Main Results:

  • Identification of novel chemistries enhancing reaction efficiency.
  • Examples of highly sensitive signal amplification strategies.
  • Demonstration of innovative signal generation mechanisms for detection.

Conclusions:

  • Oligonucleotide-templated reactions offer powerful tools for nucleic acid sensing.
  • Continued development in signal amplification and generation is key.
  • These reactions hold significant promise for diagnostics and molecular biology research.