Related Experiment Video
Updated: May 24, 2026

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
Published on: July 20, 2014
Knockdown of Sec6 improves cell-cell adhesion by increasing α-E-catenin in oral cancer cells
Toshiaki Tanaka1, Mituyoshi Iino, Kaoru Goto
1Department of Anatomy and Cell Biology, School of Medicine, Faculty of Medicine, Yamagata University, Yamagata, Japan. ttanaka@med.id.yamagata-u.ac.jp
Abstract:
The Sec6/8 complex is essential for specific exocytic sites on the plasma membrane and contributes to membrane growth in mammalian cells. In Madin-Darby canine kidney (MDCK) cells, E-cadherin and nectin-based adhesion complexes recruit the Sec6/8 complex to intercellular contacts. However, in cancer cells, the relationship between the Sec6/8 complex and the cell-cell adhesion proteins remains obscure. We demonstrate that the expression of α-E-catenin is increased by Sec6 siRNAs, and E-cadherin and β-catenin localize mainly at the cell-cell contact region in HSC3 cells, which were transfected with Sec6 siRNA.
Related Concept Videos
Cadherins in Tissue Organization
Cell Sorting During Development
Cell sorting plays an...
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Catenins
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Anchoring Junctions

