Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A scalable software framework for data integration in bioprocess development.

Engineering in life sciences·2020

Same author

Assessment of susceptible chemical modification sites of trastuzumab and endogenous human immunoglobulins at physiological conditions.

Communications biology·2018

Same author

Assessment of Telomere Length, Phenotype, and DNA Content.

Current protocols in cytometry·2017

Same author

International Society for the Advancement of Cytometry cell sorter biosafety standards.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2014

Same author

Natural killer T cells in advanced melanoma patients treated with tremelimumab.

PloS one·2013

Same author

Isolation of synaptic terminals from Alzheimer's disease cortex.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2012

Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026

Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026

Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026

Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026

Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026

Same journal

An automated workflow for quantifying the formation of synuclein aggregates in human dopaminergic neurons.

Methods (San Diego, Calif.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 24, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

How to develop a standard operating procedure for sorting unfixed cells.

Ingrid Schmid¹

¹David Geffen School of Medicine at UCLA, Department of Hematology/Oncology, Los Angeles, USA. schmid@mednet.ucla.edu

Methods (San Diego, Calif.)

|March 3, 2012

Summary

This summary is machine-generated.

Standard operating procedures (SOPs) ensure consistent laboratory tasks. For high-risk procedures like sorting unfixed cells, SOPs must detail safety elements based on risk assessment to mitigate hazards.

More Related Videos

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

Related Experiment Videos

Last Updated: May 24, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

Area of Science:

Laboratory safety
Cell sorting technology
Risk management in research

Background:

Standard operating procedures (SOPs) are essential for consistent laboratory task performance.
High-risk procedures, such as sorting unfixed cells with jet-in-air sorters, necessitate robust safety protocols within SOPs.
Effective SOPs for cell sorting require a thorough risk assessment of potential hazards.

Purpose of the Study:

To review the hazards associated with sorting unfixed cells.
To outline the process for developing comprehensive safety elements in SOPs for cell sorting.
To guide the selection of facility design, instrument placement, safety equipment, and practices.

Main Methods:

Review of existing literature and safety guidelines related to cell sorting.
Analysis of hazards inherent in sorting unfixed cells.
Framework development for risk assessment in SOP creation.

Main Results:

Identification of key safety considerations for cell sorting SOPs, including sample handling, equipment setup, validation, operation, troubleshooting, maintenance, PPE, and training.
Emphasis on the critical role of risk assessment in defining appropriate safety measures.
Guidance on integrating facility design, instrument placement, and operational practices for enhanced safety.

Conclusions:

Comprehensive SOPs incorporating detailed safety elements are crucial for high-risk laboratory procedures like unfixed cell sorting.
A proactive approach based on thorough risk assessment is fundamental to ensuring operator and laboratory safety.
The review provides a foundational understanding for creating effective safety protocols in cell sorting environments.