[Activity and properties of L-lysine amidase in Salmonella strains]

Distribution, activity level and properties of alpha-lysinamidase have been studied in Salmonella strains. The Km value for L-lysinamide was calculated to be 4.2 mM and for L-alpha-aminocaprolactame--5.1 mM. This enzyme, parallel with lysinamide, catalyzes hydrolysis of alpha-aminocaprolactam and leucinamide. Asparagine, glutamine, caprolactam, triptophanamide were not lysinamidase substrates.