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Updated: May 24, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Derepression of carbon catabolite repression in an extractive liquid-surface immobilization (Ext-LSI) system
Shinobu Oda1, Hiromi Araki, Shinichi Ohashi
1Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan. odas@neptune.kanazawa-it.ac.jp
Abstract:
An extractive liquid-surface immobilization (Ext-LSI) system with a fungal mat formed on the surface of a liquid medium effectively enabled derepression of carbon catabolite repression. In this system, a fungicidal secondary metabolite 6-pentyl-α-pyrone was efficiently produced by Trichoderma atroviride AG2755-5NM398 despite the addition of 25% glucose or fructose.
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