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Related Experiment Video

Updated: May 24, 2026

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Published on: July 12, 2018

Mouse background strain profoundly influences Paneth cell function and intestinal microbial composition.

Ajay S Gulati¹, Michael T Shanahan, Janelle C Arthur

¹Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, North Carolina, United States of America. agulati@med.unc.edu

|March 3, 2012

Summary

This summary is machine-generated.

Host genotype significantly impacts Paneth cell function and gut microbiota composition. Different mouse strains exhibit distinct antimicrobial peptide profiles and bacterial communities, influencing host-microbial interactions.

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Published on: December 16, 2021

Area of Science:

Immunology
Microbiology
Genetics

Background:

Paneth cells are crucial for intestinal host-microbial homeostasis.
The influence of host genotype on Paneth cell function is not well understood.
This study compares Paneth cell function and microbiota in C57BL/6 and 129/SvEv mouse strains.

Purpose of the Study:

To investigate the impact of host genotype on Paneth cell function.
To analyze differences in intestinal microbial composition between mouse strains.
To identify specific antimicrobial peptides and microbial alterations.

Main Methods:

Flow cytometry for Paneth cell enumeration.
Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), acid urea-polyacrylamide gel electrophoresis, and mass spectrometry for antimicrobial peptide (AMP) expression analysis.
Reciprocal colonization of germ-free mice and terminal restriction fragment length polymorphism analysis/16S qPCR for microbial composition assessment.

Main Results:

129/SvEv mice had fewer Paneth cells and a different AMP profile compared to C57BL/6 mice.
Novel 129/SvEv α-defensin peptides were identified.
Host genotype significantly altered the intestinal microbiota; 129/SvEv mice showed expansion of pro-inflammatory E. coli with attenuated defensin activity.

Conclusions:

Host genotype critically influences Paneth cell function and intestinal microbiota composition.
Identified strain-specific alterations in AMPs and microbes are relevant to disorders like obesity and intestinal inflammation.
Findings are vital for future studies on Paneth cells, microbiota, and host health in different murine models.