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Association between two polymorphisms in the HLA-G gene and angiographic coronary artery disease

Chiara Boiocchi1, Sara Bozzini, Michele Zorzetto

  • 1Laboratory of Immunogenetics, Department of Genetics and Microbiology, University of Pavia, Pavia, Italy. chiara.boiocchi@unipv.it

Molecular Medicine Reports
|March 8, 2012
PubMed

Insights

The human leukocyte antigen-G (HLA-G) gene

Area of Science:

  • Immunogenetics
  • Cardiovascular Disease Research

Background:

  • Atherosclerosis is a leading cause of death globally.
  • Cardiac inflammation plays a key role in atherosclerosis.
  • Human Leukocyte Antigen-G (HLA-G) gene polymorphisms may influence disease susceptibility.

Purpose of the Study:

  • To investigate the association between HLA-G gene polymorphisms (rs16375 and rs1632933) and coronary artery disease (CAD).

Main Methods:

  • Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis and real-time PCR were used.
  • Genotyping of 664 CAD patients and 345 controls for HLA-G polymorphisms.
  • Analysis of allelic, genotypic, and haplotypic frequencies.

Main Results:

  • The Ins/Ins genotype frequency of the 14-bp insertion/deletion (rs16375) polymorphism was significantly higher in CAD patients.
  • The homozygous Ins/Ins genotype was independently associated with angiographic CAD (OR 2.09, P=0.03).

Conclusions:

  • The homozygous Ins/Ins genotype of the HLA-G 14-bp insertion/deletion polymorphism is a novel risk factor for coronary artery disease.
  • This finding contributes to understanding the genetic basis of this multifactorial inflammatory condition.

Keywords:
human leukocyte antigen Gpolymorphismscoronary artery diseaseimmunogenetics

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