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Updated: May 24, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core
Jose Carlos Valle-Casuso1, Francesca Di Nunzio, Yang Yang
1Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.
Abstract:
TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.
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