Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Disentangling high harmonic generation from surface and bulk states of a topological insulator.

Nature communications·2026

Same author

Terahertz-Assisted Multiband High-Harmonic Spectroscopy.

Physical review letters·2026

Same author

The removal effects and mechanism of sulfate from acid mine drainage using fly ash and sunflower heads composite biochar.

Environmental geochemistry and health·2026

Same author

Demonstration of thermal fracture limits of gas-cooled Nd:APG-1 and Tm:YLF slabs.

Optics express·2026

Same author

Intravenous Sedation-Analgesia versus Local Anesthesia for Patient Comfort and Pain Control During Interventional Procedures: A Retrospective Cohort Study.

Drug design, development and therapy·2026

Same author

Enable preclinical, high-precision image-guided radiotherapy research with a stand-alone small animal IGRT system.

Medical physics·2026

Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026

Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026

Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026

Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026

Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026

Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 24, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Imaging ultrafast molecular dynamics with laser-induced electron diffraction.

Cosmin I Blaga¹, Junliang Xu, Anthony D DiChiara

¹Department of Physics, The Ohio State University, Columbus, Ohio 43210, USA. cblaga@mps.ohio-state.edu

|March 9, 2012

Summary

This summary is machine-generated.

Laser-induced electron diffraction (LIED) enables ultrafast molecular imaging. This new method captures atomic structural changes in molecules with femtosecond precision, advancing molecular dynamics studies.

More Related Videos

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Related Experiment Videos

Last Updated: May 24, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Area of Science:

Physics, Chemistry, and Biology
Ultrafast Science
Molecular Imaging

Background:

X-ray and electron diffraction are key for determining molecular structure.
Current diffraction methods are limited to picosecond timescales.
Femtosecond sources offer potential for ultrafast molecular snapshots.

Purpose of the Study:

To demonstrate laser-induced electron diffraction (LIED) for molecular imaging.
To achieve sub-ångström precision and femtosecond exposure times.
To image molecular structural dynamics.

Main Methods:

Utilizing laser-ionized bursts of coherent electron wave packets for self-interrogation.
Applying LIED to oxygen and nitrogen molecules using mid-infrared wavelengths (1.7, 2.0, 2.3 μm).
Extracting diffraction patterns from photoelectron momentum distributions.

Main Results:

Achieved sub-ångström spatial resolution and few-femtosecond exposure times.
Demonstrated sensitivity to measure a 0.1 Å displacement in oxygen bond length within ~5 fs.
Showcased wavelength variation as a means to capture snapshots at different times.

Conclusions:

LIED is a promising technique for imaging gas-phase molecules.
Offers unprecedented spatio-temporal resolution for molecular dynamics.
Enables detailed study of ultrafast structural changes in molecules.