Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Potentiometry: Overview01:06

Potentiometry: Overview

Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathway-Aware Template-Based Retrosynthesis.

Journal of chemical information and modeling·2026
Same author

Revisiting the origin of electrochemical activity in the topological semimetal PtGa.

Chemical science·2026
Same author

Combining Ag(II) and Ag(I) Reactivity Enables Electrophotochemical Acyl Fluoride Installation on (Hetero)Arenes.

Journal of the American Chemical Society·2026
Same author

Visible-light-induced chlorine photoelimination from acridinium-phosphine gold(iii) complexes.

Chemical science·2026
Same author

Correction to "Near-Unity Triplet Quantum Yield in a Molecular Cofacial H-Dimer".

Journal of the American Chemical Society·2026
Same author

Structured Electrodes Induce Local pH as a Primary Determinant of CO<sub>2</sub> Reduction Selectivity.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: May 24, 2026

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
08:09

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins

Published on: January 7, 2019

Realization of a salt bridge-free microfluidic reference electrode.

E Victoria Dydek1, Montana V Petersen, Daniel G Nocera

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Lab on a Chip
|March 9, 2012
PubMed
Summary

This study introduces a novel microfluidic electrochemical cell featuring a true silver/silver chloride reference electrode. This design eliminates the need for salt bridges by utilizing micro-channel diffusion, enabling new electrochemical applications.

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Related Experiment Videos

Last Updated: May 24, 2026

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
08:09

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins

Published on: January 7, 2019

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Area of Science:

  • Electrochemistry
  • Microfluidics
  • Analytical Chemistry

Background:

  • Traditional electrochemical cells often require salt bridges or physical barriers for reference electrodes.
  • These components can introduce complexities, limitations, and potential contamination in microfluidic systems.

Purpose of the Study:

  • To design and demonstrate a microfluidic electrochemical cell with an integrated true silver/silver chloride (Ag/AgCl) reference electrode.
  • To overcome the limitations of conventional reference electrode designs in microfluidic applications.

Main Methods:

  • Development of a microfluidic cell in polydimethylsiloxane (PDMS).
  • Integration of a true Ag/AgCl reference electrode without physical barriers or salt bridges.
  • Utilizing slow diffusion in micro-channels to maintain reference electrode stability.
  • Demonstration using the iridium(IV)/iridium(III) redox couple.

Main Results:

  • Successful implementation of a microfluidic cell with a stable Ag/AgCl reference electrode.
  • Demonstration of the device's functionality with a model redox system.
  • Identification of operational limits through scaling analysis.

Conclusions:

  • The proposed microfluidic electrochemical cell design offers a simplified and robust alternative to traditional setups.
  • This technology has potential applications in miniaturized analytical devices and electrochemical sensing.
  • Further research can explore optimization and application in diverse electrochemical analyses.