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Updated: May 24, 2026
Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration
Published on: June 7, 2014
Effects of adipose-derived mesenchymal cells on ischemia-reperfusion injury in kidney
Kengo Furuichi1, Hidemi Shintani, Yoshio Sakai
1Division of Blood Purification, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa 920-8641, Japan. kfuruichi@m-kanazawa.jp
Background:
Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Methods:
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10(5) cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
Results:
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Conclusion:
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.
Insights
Adipose-derived mesenchymal cells show promise in treating acute kidney injury (AKI). Repeated administration reduced kidney damage and inflammation in a mouse model, suggesting potential as a cell-based therapy for ischemic kidney injury.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Nephrology
Background:
- Acute kidney injury (AKI) poses significant risks to kidney and lung health.
- Current treatment options for AKI are limited.
- This study investigates adipose-derived mesenchymal cells (ADMCs) for AKI treatment.
Purpose of the Study:
- To evaluate the therapeutic potential of ADMCs in a mouse model of ischemic AKI.
- To determine the effect of ADMCs on kidney tissue damage and inflammation.
- To explore the homing and differentiation capabilities of ADMCs.
Main Methods:
- ADMCs were isolated from mouse adipose tissue.
- Ischemic AKI was induced in C57BL/6 mice by clamping renal artery and vein.
- ADMCs or PBS were administered intravenously on days 0, 1, and 2.
Main Results:
- ADMCs exhibited stem cell markers and multilineage differentiation potential.
- Administered ADMCs primarily homed to the lung.
- Repeated ADMC administration reduced acute tubular necrosis and macrophage infiltration in kidneys, decreasing cytokine and chemokine expression.
Conclusions:
- ADMCs demonstrate therapeutic effects in a mouse model of ischemic AKI.
- ADMCs hold potential as a cell-based therapy for kidney injury.
- Further research is warranted to explore ADMC mechanisms in AKI treatment.
