[Impact of adipose-derived stem cells combined with vascular bundle implantation on vascularized tissue engineering scaffolds in vivo]
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Updated: May 24, 2026
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
Published on: May 30, 2016
Wenchen Ji1, Pei Yang, Yuelin Zhang
1No. 1 Department of Orthopedics, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an Shaanxi, 710004, PR China.
Objective:
To discuss the impact of adipose-derived stem cells (ADSCs) combined with vascular bundle implantation on vascularized tissue engineering scaffolds in vivo so as to provide a theoretical basis for the repair of avascular necrosis of the femoral head.
Methods:
ADSCs were isolated from 4-month-old Sprague Dawley (SD) rats and cultured, then were induced to osteogenesis and identified. ADSCs at the 3rd passage were seeded on the nano-hydroxyapatite/polyamide-66 (nHA/PA66) to prepare the composite scaffolds. The compound condition of cells and scaffold materials were observed under scanning electronic microscope (SEM). Twenty-four 4-month-old SD rats (weighing 350-400 g) were randomly divided into 3 groups (n=8). In group A and group B, the inferior epigastric artery and vein of rats were implanted into composite scaffold cultured for 10 days or simple nHA/PA66 scaffold, respectively. In group C, two composite scaffolds cultured for 10 days were embedded into quadriceps femoris muscle of both thighs, respectively. After 2 and 4 weeks of operation, angiogenesis was observed by HE staining and CD34 immunohistochemical staining.
Results:
Cells isolated from adipose were identified as ADSCs. SEM showed that the number of cells increased after being cultured for 10 days, cell morphology stretched fully with a shape of long spindle. HE staining and immunohistochemical staining showed that a large number of angiogenesis was observed around the implanted artery and vein in group A, which was superior to groups B and C in the number of blood vessels and the maturity of blood vessel wall. After 2 and 4 weeks of operation, the blood vessel density and blood vessel diameter were significantly higher in group A than in group B and group C, and in group B than in group C (P < 0.05).
Conclusion:
Combined application of ADSCs and vascular bundle implantation can promote the degree of vascularization, which could make the scaffold vascularization reliable.
