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Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Published on: July 1, 2014
Comparative evaluation of two isobaric labeling tags, DiART and iTRAQ
Zhen Chen1, Quanhui Wang, Liang Lin
1Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
Analytical Chemistry
|March 13, 2012
Summary
Deuterium isobaric amine reactive tag (DiART) offers improved protein quantification accuracy in complex samples compared to isobaric tag for relative and absolute quantitation (iTRAQ). DiART provides stronger signals and less ratio compression, enhancing quantitative proteomics.
Area of Science:
- Proteomics
- Mass Spectrometry
- Biotechnology
Background:
- Isobaric tags are crucial for quantitative proteomics, with isobaric tag for relative and absolute quantitation (iTRAQ) being widely used.
- Recent studies highlight potential biases in iTRAQ quantification within complex biological samples.
Purpose of the Study:
- To evaluate deuterium isobaric amine reactive tag (DiART) as an alternative to iTRAQ for quantitative proteomics.
- To compare the performance of DiART and iTRAQ using the proteome of Thermoanaerobacter tengcongensis.
Main Methods:
- Comparative analysis of DiART and iTRAQ labeling strategies.
- Assessment of fragmentation mechanisms, protein identification, and quantification accuracy.
- Utilized identical chromatography and mass spectrometry conditions for direct comparison.
Main Results:
- DiART produced significantly stronger reporter ions than iTRAQ.
- DiART improved signal-to-noise ratio (S/N) without reducing peptide identification.
- DiART exhibited less reporter ion ratio compression compared to iTRAQ.
Conclusions:
- DiART demonstrates enhanced performance for quantitative proteomics.
- DiART is a valuable alternative to iTRAQ, offering improved accuracy and signal intensity.
- The study highlights DiART's potential for complex proteomic analyses.
