Branching structure and chain conformation of water-soluble glucan extracted from Auricularia auricula-judae

A water-soluble neutral polysaccharide (AF1) was extracted from Auricularia (A.) auricula-judae with 0.15 M aqueous NaCl at 80-100 °C. Its chemical components and structure were analyzed by GC, GC-MS, and NMR. AF1 was identified as a β-(1→3)-D-glucan with two β-(1→6)-D-glucosyl residues for every three main chain glucose residues, showing a comb-branched structure. The M(w) values of AF1 in both aqueous solution and DMSO determined by LLS and SEC-LLS were in the narrow range of 2.07-2.15 × 10(6), indicating AF1 existed as single chains in the two solvents. The high intrinsic viscosity [η] of 1753 mL/g and the structure-sensitive parameter ρ (≡R(g)/R(h)) value of 2.3 in water revealed that AF1 existed as stiff chain conformation. Moreover, we directly observed the extended stiff chain conformation by AFM. The branching structure led to the water solubility of AF1, and the intramolecular hydrogen bonds sustained the stiff chain conformation. The rheological results showed that this polysaccharide aqueous solution had higher viscosity than even xanthan, a pronounced thickening agent. This work provided important information for developing new thickeners in food fields, and how neutral polysaccharides can be used as good candidates.