Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 24, 2026

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

Imaging calcium in neurons.

Christine Grienberger1, Arthur Konnerth

  • 1Institute of Neuroscience, Technical University Munich, Biedersteinerstr. 29, 80802 Munich, Germany.

Neuron
|March 13, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An unsuspected physiological role for mGluRIII glutamate receptors in hippocampal area CA1.

Cell reports·2026
Same author

Thalamic activation of the visual cortex at the single-synapse level.

Science (New York, N.Y.)·2026
Same author

Subicular Plateaus Signal Reward Locations during Goal-Directed Behavior.

bioRxiv : the preprint server for biology·2026
Same author

Cellular multipoint adaptive technology for two-photon mesoscope.

Neurophotonics·2026
Same author

Learning-dependent feedback by OLM interneurons shapes CA1 representations.

bioRxiv : the preprint server for biology·2026
Same author

Glutamate indicators with increased sensitivity and tailored deactivation rates.

Nature methods·2025
Same journal

Dynamic coordination and segregation mechanisms in higher cortex for parallel task processing.

Neuron·2026
Same journal

Higher-order thalamic bursts are drivers of attention control.

Neuron·2026
Same journal

Composing trajectories for rapid inference of navigational goals.

Neuron·2026
Same journal

Peri-head distance coding in the mouse brainstem.

Neuron·2026
Same journal

A two-timepoint framework for sensitive and specific single-cell activity screening.

Neuron·2026
Same journal

From first impressions to bonds: The neural dynamics of social relationships.

Neuron·2026
See all related articles

This primer explains neuronal calcium signaling and imaging techniques. It covers advanced methods like in vivo imaging in behaving animals for studying neural circuits and plasticity.

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biophysics

Background:

  • Calcium ions (Ca2+) are crucial intracellular signals regulating neuronal functions.
  • Understanding neuronal Ca2+ signaling is key to deciphering brain activity.
  • Specific Ca2+ dynamics occur within distinct neuronal subcompartments.

Purpose of the Study:

  • To provide a comprehensive overview of neuronal calcium signaling mechanisms.
  • To introduce various calcium imaging technologies and indicators.
  • To highlight recent advancements and future prospects in neuronal calcium imaging.

Main Methods:

  • Review of general mechanisms of neuronal calcium signaling.
  • Introduction to calcium imaging devices: confocal microscopy, two-photon microscopy, and miniaturized devices for freely moving animals.

More Related Videos

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique
11:41

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

Published on: April 8, 2022

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Related Experiment Videos

Last Updated: May 24, 2026

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique
11:41

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

Published on: April 8, 2022

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

  • Overview of calcium indicators: chemical fluorescent and genetically encoded.

    • Main Results:

    • Discussion of classical and advanced calcium indicators.
    • Demonstration of new developments, including in vivo imaging in awake, behaving animals.
    • Application examples: mapping sensory inputs in cortical neurons.

    Conclusions:

    • Calcium imaging is essential for analyzing neuronal signaling and plasticity.
    • Technological advancements enable sophisticated studies of neural circuits in vivo.
    • Future research will leverage these techniques across diverse animal models.