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Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

Double Resonance Techniques: Overview

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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Mass Analyzers: Overview

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Related Experiment Video

Updated: May 24, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

Using high-resolution quadrupole TOF technology in DMPK analyses.

J Larry Campbell¹, J C Yves Le Blanc

¹AB Sciex, Concord, Ontario, Canada.

|March 14, 2012

Summary

This summary is machine-generated.

Quadrupole time-of-flight (Q-TOF) mass spectrometry offers a paradigm shift, potentially replacing triple-quadrupole MS for high-throughput bioanalysis. Modern Q-TOF instruments with advanced features are evaluated for their readiness in drug metabolism and pharmacokinetics workflows.

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Published on: April 17, 2018

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Last Updated: May 24, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

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Published on: August 6, 2018

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Area of Science:

Analytical Chemistry
Mass Spectrometry
Bioanalysis

Background:

Recent advancements in quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) are prompting a reevaluation of established bioanalytical workflows.
There is a potential shift away from traditional triple-quadrupole MS methods towards Q-TOF platforms.

Purpose of the Study:

To assess the readiness of modern Q-TOF-MS instruments for high-throughput bioanalytical applications, particularly in drug metabolism and pharmacokinetics (DMPK).
To explore the potential for merging quantitation and qualification studies within a single Q-TOF workflow.

Main Methods:

Review of recent technological advancements in Q-TOF MS.
Examination of orthogonal technologies, such as ion mobility, integrated into Q-TOF systems.
Evaluation of Q-TOF performance metrics including mass accuracy (≤5 ppm) and resolving power (≥30,000).

Main Results:

Q-TOF instruments offer high data acquisition rates, excellent mass accuracy, and high resolving power, enabling more generic workflows.
These capabilities present significant advantages for high-throughput analyses, including potential for combined quantitation and qualification in DMPK studies.
Orthogonal technologies like ion mobility further enhance the analytical power of Q-TOF systems.

Conclusions:

Modern Q-TOF-MS instruments are approaching readiness for a paradigm shift in bioanalysis, offering enhanced efficiency and merged workflows.
Further integration and validation of Q-TOF technology, potentially with ion mobility, will be crucial for widespread adoption in high-throughput DMPK analysis.