Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
Vaccines01:21

Vaccines

Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the type of...
Viral Structure00:56

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocadherin-1 deficiency increases baseline and allergen-induced airway hyperresponsiveness in mice.

American journal of respiratory cell and molecular biology·2026
Same author

The Ste20 kinase TAOK3 restrains Rac-driven cytoskeletal-mitochondrial coupling to preserve naive CD8<sup>+</sup> T cell homeostasis and activation.

Frontiers in immunology·2026
Same author

Endothelial cells notch monocytes toward an alveolar macrophage fate.

The Journal of experimental medicine·2026
Same author

Multivalent Antibody-Recruiting Molecules for Targeted Immune-Killing of Carbonic Anhydrase IX-Expressing Cancer Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Marker-Agnostic Tumor Anchoring Chimeras Enable pH-Gated Immune Engagement.

Angewandte Chemie (International ed. in English)·2026
Same author

Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103<sup>+</sup> cDC1s and CD301b<sup>+</sup> cDC2s.

STAR protocols·2026

Related Experiment Video

Updated: May 24, 2026

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

Surface-engineered polyelectrolyte multilayer capsules: synthetic vaccines mimicking microbial structure and

Bruno G De Geest1, Monique A Willart, Bart N Lambrecht

  • 1Laboratory of Pharmaceutical Technology, Department of Pharmaceutics, Ghent University, Belgium. br.degeest@ugent.be

Angewandte Chemie (International Ed. in English)
|March 14, 2012
PubMed
Summary
This summary is machine-generated.

New hollow capsules effectively deliver antigens to dendritic cells, triggering potent immune responses for enhanced immunization strategies. This novel approach utilizes layered dextran/poly-L-arginine capsules coated with CpG oligonucleotides.

More Related Videos

Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
07:44

Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen

Published on: May 12, 2023

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform
06:27

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

Published on: October 20, 2020

Related Experiment Videos

Last Updated: May 24, 2026

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
07:44

Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen

Published on: May 12, 2023

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform
06:27

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

Published on: October 20, 2020

Area of Science:

  • Immunology
  • Biomaterials Science
  • Nanotechnology

Background:

  • Developing effective antigen delivery systems is crucial for potent vaccine-induced immunity.
  • Dendritic cells are key antigen-presenting cells that initiate adaptive immune responses.
  • Layer-by-layer assembly offers a versatile platform for creating functional nanostructures.

Purpose of the Study:

  • To engineer novel hollow capsules for efficient antigen delivery and immune stimulation.
  • To evaluate the immunogenicity of antigen-loaded capsules coated with immune-activating CpG oligonucleotides.
  • To assess the uptake of these capsules by dendritic cells.

Main Methods:

  • Fabrication of hollow capsules using layer-by-layer assembly of dextran sulphate and poly-L-arginine.
  • Encapsulation of ovalbumin as a model antigen within the capsules.
  • Coating the capsules with CpG-containing oligonucleotides to enhance immune activation.
  • In vitro assessment of dendritic cell uptake and in vivo immunization experiments.

Main Results:

  • The fabricated hollow capsules successfully encapsulated the model antigen ovalbumin.
  • Capsules coated with CpG oligonucleotides were readily internalized by dendritic cells.
  • These engineered capsules demonstrated significant activity in subsequent immunization experiments, indicating potent immune responses.

Conclusions:

  • Layered dextran/poly-L-arginine hollow capsules represent a promising platform for antigen delivery.
  • Coating with CpG oligonucleotides enhances cellular uptake and immunogenicity.
  • This nanotechnology-based approach holds potential for developing next-generation vaccines and immunotherapies.