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Updated: Jun 23, 2026

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Endothelial cells notch monocytes toward an alveolar macrophage fate
Stijn Verwaerde1,2, Bart N Lambrecht1,2,3, Martin Guilliams4,5
1Laboratory of Immunoregulation and Mucosal Immunology, VIB-UGent Center for Inflammation Research, Ghent, Belgium.
Abstract:
Mínguez-Martínez et al. (https://doi.org/10.1084/jem.20242085) identify a cooperative RBPJ-STAT5-SMAD4-RXRα-PPARγ transcriptional network that integrates DLL4, GM-CSF, and TGFβ signals to establish alveolar macrophage identity. Their findings position endothelial DLL4 as early licensing signal that enables recruited monocytes to acquire tissue-resident macrophage fate.
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