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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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[ESBL producing organisms].

Yoshikazu Ishii1

  • 1Department of Microbiology and Infectious Diseases, Toho University School of Medicine.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|March 15, 2012
PubMed
Summary

Extended-spectrum beta-lactamase (ESBL) producing Escherichia coli, particularly the ST131 clone, is rising globally. Current evidence does not directly link ESBL spread between humans and animals or food sources.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Epidemiology

Background:

  • Extended-spectrum beta-lactamase (ESBL) producing organisms, especially CTX-M-15 producing Escherichia coli, are increasingly prevalent worldwide.
  • The O25b:H4-ST131 clone is the predominant ESBL-producing E. coli epidemic clone in humans.
  • ESBL producers are found in various sources, including infected patients, healthy individuals, domestic animals, food, and the environment.

Purpose of the Study:

  • To investigate the prevalence and characteristics of ESBL-producing E. coli, focusing on the ST131 clone.
  • To determine potential transmission routes of ESBL producers between humans, domestic animals, and food.

Main Methods:

  • Surveillance and isolation of ESBL-producing E. coli from diverse sources.

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  • Molecular typing and characterization of isolated strains, including CTX-M subgroup analysis.
  • Comparative analysis of E. coli ST131 and other ESBL producers across different sample types.

    • Main Results:

    • While ESBL producers are widespread, the E. coli ST131 clone is rarely isolated from domestic animals or food.
    • CTX-M-1 and CTX-M-9 are the dominant ESBL subgroups in human isolates.
    • The CTX-M-2 subgroup is predominant in non-human samples.

    Conclusions:

    • There is a lack of direct evidence for the transmission of ESBL producers between humans and domestic animals or food sources.
    • The distinct CTX-M profiles in human versus non-human isolates suggest separate evolutionary or epidemiological pathways.
    • Further research is needed to fully understand the ecology and transmission dynamics of ESBL-producing E. coli.