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Updated: May 24, 2026

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
Published on: September 8, 2023
Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films
Matteo Savoini1, Xiaofei Wu, Michele Celebrano
1CNISM-Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci, 20133 Milano, Italy.
Abstract:
Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.
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