Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
Polyprotic Acids03:38

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary Intake of Essential Elements from African Foods Available in the UK Market.

Foods (Basel, Switzerland)·2026
Same author

Natural allelic variation in rice reveals root-centric transcriptome and haplotype networks underpinning arsenic tolerance for food safety.

Journal of hazardous materials·2026
Same author

QTL qLDC5 regulates primary root branching in an auxin-dependant manner.

Journal of experimental botany·2026
Same author

Genetic variation in leaf bronzing and dry matter production of rice varieties as indicators of tolerance to iron toxicity.

Breeding science·2026
Same author

Ozone-Tolerant Rice for Air-Polluted Environments.

Global change biology·2025
Same author

Low-call-rate SNPs and presence-absence variation identified in the rice pan-genome can improve genomic prediction of rice gene bank accessions.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2025

Related Experiment Video

Updated: May 24, 2026

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
10:22

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Published on: June 30, 2020

Leaf ascorbic acid level--is it really important for ozone tolerance in rice?

Michael Frei1, Matthias Wissuwa, Juan Pariasca-Tanaka

  • 1Institute of Crop Science and Resource Conservation-INRES-Plant Nutrition, University of Bonn, Karlrobert-Kreiten Strasse 13, 53115 Bonn, Germany. mfrei@uni-bonn.de

Plant Physiology and Biochemistry : PPB
|March 16, 2012
PubMed
Summary

Lower ascorbic acid (ASA) in rice plants increased ozone damage and reduced yield under high ozone conditions. This suggests ASA levels are crucial for developing ozone-tolerant crops.

More Related Videos

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
05:44

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Published on: November 25, 2022

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Related Experiment Videos

Last Updated: May 24, 2026

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
10:22

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Published on: June 30, 2020

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
05:44

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Published on: November 25, 2022

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Area of Science:

  • Plant Science
  • Agricultural Science
  • Biochemistry

Background:

  • Ascorbic acid (ASA) is vital for plant stress tolerance, but its role in crop breeding is not fully understood.
  • Ozone (O3) is a significant environmental stressor impacting crop yield and quality.

Purpose of the Study:

  • To investigate the role of leaf ascorbic acid (ASA) in ozone tolerance in rice (Oryza sativa L.).
  • To assess the impact of reduced ASA levels on plant physiology, yield, and crop quality under ozone stress.

Main Methods:

  • Utilized a rice TOS17 insertion mutant (ND6172) with reduced ASA biosynthesis and compared it to the wild-type (Nipponbare).
  • Exposed rice plants to controlled fumigation experiments with varying ozone concentrations and durations.
  • Measured physiological parameters including ASA levels, antioxidant enzyme activities, visible leaf damage, yield, and straw lignification.

Main Results:

  • The ND6172 mutant showed 20-30% lower ASA levels and increased visible leaf damage under ozone exposure.
  • Differential responses in glutathione levels and antioxidant enzyme activities were observed between genotypes.
  • Yield reductions and increased straw lignification were more pronounced in the ND6172 mutant under elevated ozone concentrations.

Conclusions:

  • A 20-30% difference in leaf ASA levels significantly impacts ozone tolerance in rice.
  • Reduced ASA levels compromise plant defense mechanisms against ozone, leading to yield losses at elevated ozone concentrations.
  • Targeting ASA levels could be a viable strategy for breeding ozone-tolerant rice varieties.