Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 24, 2026

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Towards an efficient computational mining approach to identify EST-SSR markers.

Jagajjit Sahu, Priyabrata Sen, Manabendra Dutta Choudhury

    Bioinformation
    |March 16, 2012
    PubMed
    Summary

    Developing SSR markers from ESTs computationally is efficient. This in silico approach for microsatellite marker development saves time and cost, aiding genetic variability studies and marker-assisted selection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Correction: Nanotechnology-enhanced Natural Products for Cancer Chemoprevention: Molecular Mechanisms and Clinical Translation.

    The AAPS journal·2026
    Same author

    Cephalosporin Exposure Influences Transcriptional Response of mrcA and mrcB in Escherichia coli Harbouring bla<sub>CTX-M</sub>.

    Current microbiology·2026
    Same author

    Integrative in-silico analysis on phytocompounds of <i>Pajanelia longifolia</i> against hepatocellular carcinoma: gene network target identification, molecular docking, simulation and ADMET profiling.

    In silico pharmacology·2026
    Same author

    Nanotechnology-enhanced Natural Products for Cancer Chemoprevention: Molecular Mechanisms and Clinical Translation.

    The AAPS journal·2026
    Same author

    Exclusion versus detoxification: contrasting molecular strategies of aluminium tolerance in rice landraces of Northeast India.

    Plant cell reports·2026
    Same author

    Linking topography, soil physicochemical traits and bacterial community dynamics in a managed tea landscape.

    International microbiology : the official journal of the Spanish Society for Microbiology·2026

    Area of Science:

    • Genomics
    • Molecular Biology
    • Bioinformatics

    Background:

    • Microsatellites, or Simple Sequence Repeats (SSRs), are valuable genetic markers due to their abundance, reproducibility, polymorphism, and co-dominant nature.
    • They are crucial for assessing genetic variability within species and for marker-assisted selection in breeding programs.
    • Expressed Sequence Tags (ESTs) are a primary source for identifying SSRs.

    Purpose of the Study:

    • To provide an overview of the in silico approach for developing SSR markers from EST sequences.
    • To highlight the efficiency and advantages of computational methods over traditional techniques.
    • To introduce freely available tools for academic use in EST-SSR marker development.

    Main Methods:

    • Utilizing computational tools and web interfaces for analyzing EST sequence databases.
    Keywords:
    BioinformaticsEST-SSRMicrosatellitesSSR Markersin silico approach

    More Related Videos

    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
    13:47

    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

    Published on: February 24, 2015

    Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
    11:13

    Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

    Published on: March 12, 2020

    Related Experiment Videos

    Last Updated: May 24, 2026

    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
    10:27

    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

    Published on: April 20, 2018

    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
    13:47

    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

    Published on: February 24, 2015

    Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
    11:13

    Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

    Published on: March 12, 2020

  • Employing in silico methods to detect SSRs within EST sequences.
  • Developing EST-SSR markers through bioinformatic analysis.

    • Main Results:

    • The computational approach significantly reduces the time and cost associated with SSR marker development.
    • Various efficient, freely available tools facilitate the analysis of EST sequences for marker discovery.
    • This in silico strategy enables the identification of potential EST-SSR markers.

    Conclusions:

    • In silico development of SSR markers from ESTs is a cost-effective and time-efficient strategy.
    • Bioinformatic tools provide a powerful platform for discovering novel SSR markers.
    • This approach supports genetic research, species characterization, and marker-assisted selection.