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Development of a high performance liquid chromatography method for the simultaneous measurement of prednisone and
F Huber1, M Wiedemann, G Heinrich
1Analytical Research Centre of the LAB GmbH, Neu Ulm, Fed. Rep. of Germany.
Arzneimittel-Forschung
|August 1, 1990
Abstract:
An improved high performance liquid chromatographic assay (HPLC) is presented for the quantitative determination of prednisone and its main metabolite prednisolone. This HPLC assay, employing a UV-detector and dexamethasone as an internal standard, provides a selective and sensitive determination of glucocorticoids in plasma. The advantage of this method is the possibility of simultaneously measuring exogenic and endogenic glucocorticoids. The lower limit of quantification is 5 ng/ml.