Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechano-immunomodulation of macrophages influences the regenerative environment of fracture healing through the regulation of angiogenesis and osteogenesis.

Acta biomaterialia·2025
Same author

Human macrophage polarisation and regulation of angiogenesis and osteogenesis is dependent on culture extracellular matrix and dimensionality.

Biochemical and biophysical research communications·2024
Same author

Feature size specific processing parameters for additively manufactured Ti-6Al-4V micro-strut lattices.

Journal of the mechanical behavior of biomedical materials·2024
Same author

One-stage surgical case management of a two-year-old Arabian horse affected by male-pseudo hermaphroditism.

Journal of equine veterinary science·2024
Same author

Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large, load-bearingarticular cartilage defects.

European cells & materials·2023
Same author

Integrating melt electrowriting and inkjet bioprinting for engineering structurally organized articular cartilage.

Biomaterials·2022
Same journal

Corrigendum to "Senescent endothelial cells' response to the degradation of bioresorbable scaffold induces intimal dysfunction accelerating in-stent restenosis" [Acta Biomaterialia 166 (2023) 266-277].

Acta biomaterialia·2026
Same journal

Colorectum and embedded networks of nerve fibers present auxetic responses during uniaxial circumferential extension.

Acta biomaterialia·2026
Same journal

Music-Inspired Acoustic-Piezoelectric Stimulation Accelerates Extracellular Vesicle Production and Programs Therapeutic Function.

Acta biomaterialia·2026
Same journal

Mutant superoxide dismutase 1-catalyzed hydrogen therapy for amyotrophic lateral sclerosis achieved by intercepting oxidative stress-neuroinflammation crosstalk.

Acta biomaterialia·2026
Same journal

Injectable pH-responsive gelatin methacryloyl hydrogel for cuproptosis-synergized sunitinib therapy and immune reprogramming in clear cell renal cell carcinoma.

Acta biomaterialia·2026
Same journal

Corrigendum to "Injectable hydrogel-assisted local lipopolysaccharide delivery improves immune checkpoint blockade therapy" [Acta Biomaterialia 2025, 194, 153-168].

Acta biomaterialia·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

Cell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure.

A J Steward1, S D Thorpe, T Vinardell

  • 1Trinity Centre for Bioengineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.

Acta Biomaterialia
|March 20, 2012
PubMed
Summary
This summary is machine-generated.

Hydrostatic pressure (HP) enhances chondrogenesis in mesenchymal stem cells (MSCs) within fibrin hydrogels, but not agarose. The biomaterial influences how cells respond to HP, impacting differentiation.

More Related Videos

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Related Experiment Videos

Last Updated: May 24, 2026

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Hydrostatic pressure (HP) and cell-matrix interactions independently regulate mesenchymal stem cell (MSC) chondrogenesis.
  • Understanding their combined effects is crucial for optimizing cartilage tissue engineering.

Purpose of the Study:

  • To investigate if the response of MSCs to HP is influenced by the encapsulating biomaterial.
  • To determine the interplay between HP, cell-matrix interactions, and chondrogenic differentiation.

Main Methods:

  • Bone-marrow-derived MSCs were encapsulated in agarose or fibrin hydrogels.
  • Cells were exposed to cyclic HP (10 MPa) with TGF-β3.
  • Chondrogenesis and hydrogel properties were assessed over 3 weeks.

Main Results:

  • Agarose supported spherical morphology; fibrin promoted cell spreading and stress fibers, with hydrogel contraction.
  • HP enhanced sulfated glycosaminoglycan (sGAG) accumulation in fibrin, correlating with reduced contraction.
  • HP reduced alkaline phosphatase activity in both hydrogel types.

Conclusions:

  • Biomaterial choice significantly modulates MSC response to HP during chondrogenesis.
  • HP's chondrogenic benefits in fibrin are linked to reduced matrix remodeling.
  • A complex interaction between cell-matrix and HP regulates MSC differentiation.